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Natural logarithm of the observed unfolding equilibrium constant Kobs for thermal denaturation of the 5′-r(GAUAGUAGAUAG)-3′ duplex as a function of glycine betaine molality at 45.5 (filled circles), 47.1 (open circles), 48.6 (filled diamonds), 50.2 (open diamonds), and 51.7 °C (filled squares). Linear regression slopes are equal to − Δμ23,4/RT. Error bars on ln Kobs are smaller than symbols.
