Table 1.

Glycine Betaine Δμ_23,4/RT and m-Values with RNA Duplex Dodecamers During Thermal Denaturation

sequence	%GC	T/°Ca	Δμ23,4/RT/m−1b	m-value/kcal mol−1 m−1b	d(m-value)/dT/kcal mol−1 m−1 K−1	dΔHobso/dm3/kcal mol−1 m−1
5′-r(GAAAUUAUAAAG)-3′	17	27.3	−0.315 ± 0.029	−0.188 ± 0.017	−0.0044 ± 0.0003	1.12 ± 0.09
5′-r(GAAAGUAUAAAG)-3′	25	34.8	−0.398 ± 0.044	−0.244 ± 0.027	−0.0065 ± 0.0002	1.76 ± 0.05
5′-r(GAUAGUAGAUAG)-3′	33	45.5	−0.598 ± 0.027	−0.378 ± 0.017	−0.0191 ± 0.0012	4.03 ± 0.18
5′-r(GAAAGUAGAAAC)-3′	33	40.4	−0.418 ± 0.036	−0.260 ± 0.022	−0.0139 ± 0.0006	5.71 ± 0.39
5′-r(GCAAAGUAAACG)-3′	42	44.6	−0.670 ± 0.069	−0.423 ± 0.044	−0.0142 ± 0.0009	4.22 ± 0.28
5′-r(GCAAAGCAAACG)-3′	50	49.0	−1.02 ± 0.07	−0.655 ± 0.045	−0.0195 ± 0.0004	5.61 ± 0.12
5′-r(GCAUAGCAUACG)-3′	50	52.0	−0.811 ± 0.032	−0.524 ± 0.020	−0.0188 ± 0.0004	5.63 ± 0.15
5′-r(GCGAAGCCAACG)-3′	67	59.6	−0.948 ± 0.037	−0.627 ± 0.024	−0.0266 ± 0.0021	8.41 ± 0.69
5′-r(GCGCCGCCGGCG)-3′	100	80.9	−1.44 ± 0.03	−1.010 ± 0.023	−0.0405 ± 0.0031	12.9 ± 1.1

^aReference temperatures for RNA duplex unfolding during thermal denaturation determined at the temperature where the fraction of unfolded duplex total strand was 0.2 in 0 m glycine betaine.

^bDetermined at reference temperature.