ITC reconstitution isotherms
in (a) the absence or (b) the presence
of 1.5 μM KcsA tetramer at 8 °C. Both isotherms depict
the heats of reaction, Q (circles), obtained upon
titration of 35 mM OG with 50 mM E. coli polar lipid
extract. Also shown are the SOL and SAT boundaries (red and blue lines,
respectively) and the uncertainty in the SAT boundary in the presence
of KcsA (light blue band). Inset: Thermogram displaying differential
heating power, Δp, versus time, t. The discontinuity at t ≈ 10 h is due to
an increase in injection volume.