Figure 4.

Size characterization of reconstituted proteoliposomes and protein-free lipid vesicles by DLS. (a) Normalized autocorrelation function, C(τ), versus delay time, τ, as determined for sonicated E. coli polar lipid vesicles before reconstitution (black) and the reconstitution mixture after calorimetrically monitored lipid addition to OG-solubilized KcsA (red). (b) Intensity-weighted distribution functions, f(d), of the hydrodynamic diameter, d, of sonicated E. coli polar lipid vesicles before reconstitution (black) and proteoliposomes after reconstitution and removal of aggregates and OG by dilution, centrifugation, and extrusion (blue).