Table 3.
Comparison of enterotoxigenic Escherichia coli detection in travelers' diarrhea samples by DNA hybridization and quantitative real-time polymerase chain reaction (qPCR) assays
| DNA hybridizationresults | LT+ ST+ 26 | LT+ ST− 21 | LT− ST+ 23 | LT− ST− 11* | LT−ST− 12† | |
|---|---|---|---|---|---|---|
| eltA+ sta1+ sta2+ | 3 | 3 | 4 | |||
| eltA+ sta1+ sta2- | 2 | 3 | 1 | 2 | ||
| qPCR | eltA+ sta1- sta2+ | 4 | 1 | |||
| Results | eltA+ sta1- sta2- | 2 | 3 | 1 | 1 | 1 |
| eltA- sta1+ sta2- | 2 | 3 | ||||
| eltA- sta1- sta2+ | 1 | 2 | ||||
| eltA- sta1- sta2- | 12 | 11 | 12 | 8 | 11 | |
| Total | 26 | 21 | 23 | 11 | 12 |
*
No pathogen identified as the diarrheal etiologic agent.
†
Samples collected from healthy travelers.
LT = heat labile enterotoxin; ST = heat stable enterotoxin.