Figure 2.
Neighbor-joining (NJ) tree derived from partial 56-kDa TSA genes (variable domains I–III, 498 bp) of O. tsutsugamushi. The analysis involved 46 nucleotide sequences of O. tsutsugamushi-infected chigger lines (Lc-1, Li-1, Ld-1), O. tsutsugamushi-infected wild-caught rodent and chigger along with reference sequences retrieved from the GenBank database as shown in Table 5. The NJ tree was constructed using the p-distance method of nucleotide substitution model. The bootstrap P values > 50% are indicated at major nodes (2,000 replicates). Tree is rooted at its midpoint. Scale bar represents substitutions per site.