Table 3.
Similarities of 56-kDa TSA gene sequences of the variable domains I–III from chiggers (Lc-1, Li-1, Ld-1) and the rodent post-infection to reference genotypes (Karp, Gilliam, UT302)
| Mouse feeding experiment | Sample | Sequence type | Sequence similarity to reference genotypes* | ||
|---|---|---|---|---|---|
| Karp (AY956315) | UT302 (EF213095) | Gilliam (DQ485289) | |||
| Lc-1 | Chigger | Lc-1 | 20/20 (94.2) | – | – |
| Mouse spleen | Lc-1 | 20/20 (94.2) | – | – | |
| Li-1 | Chigger | Li-1a | – | 12/18 (98.5) | – |
| Li-1b | 5/18 (93.2) | – | – | ||
| N/A | 1/18 (99.8) | – | – | ||
| Mouse spleen | Li-1a | – | 17/19 (98.5) | – | |
| Li-1b | 2/19 (93.2) | – | — | ||
| Ld-1 | Chigger | Ld-1a | – | – | 11/18 (93.0) |
| Ld-1b | 7/18 (93.2) | – | – | ||
| Mouse spleen | Ld-1b | 20/20 (93.2) | – | – | |
*
Number of bacterial colonies with a 56 kDa type-specific antigen (TSA) gene insert matching reference genotype/total number of colonies evaluated. Numbers in parentheses represent percent sequence similarity to corresponding reference genotype.