. 2014 Jan 8;90(1):139–146. doi: 10.4269/ajtmh.13-0393

Table 4.

Characterization of Orientia tsutsugamushi obtained from field-collected rodents and a chigger in Thailand based on 56-kDa TSA sequence homology to reference genotypes (Karp, Gilliam, UT302)

Location	Rodent species	No. of infected chiggers/total collected^*	Similarity to reference genotypes (no. of 56-kDa TSA sequences matching reference genotype/total sequences analyzed)^†
Location	Rodent species	No. of infected chiggers/total collected^*	Rodent	Chigger^‡
Buri Ram (Forest)	Bandicota indica (BA0185)	0/16	Gilliam (5/20)	–
			Karp (14/20)	–
			UT302 (1/20)	–
Chachoeng Sao (Rice field)	Bandicota indica (BA0344)	2/7^‡	Gilliam (15/15)	Gilliam (4/18)
Chachoeng Sao (Rice field)	Bandicota indica (BA0344)	2/7^‡	Gilliam (15/15)	UT302 (14/18)

Determined by Otsu47 quantitative real-time polymerase chain reaction (qPCR) assay on DNA sample extracted from an individual chigger.

^†

Number of bacterial colonies with a 56-kDa type-specific antigen (TSA) insert matching reference genotype/total number of colonies evaluated.

^‡

Characterization performed on one infected chigger.