Table 1.

Primers for STR amplification and corresponding chromosomal locations

STR marker	GenBank accession #	Location on chromosome (bp)	Primers (5′–3′)	Primer μM
18-3	NT_039674.8	60271556–60271705	F: [FAM]-TCTTTCTCCTTTTGTGTCATGC R: GTTTCTTGCTAAATAACTAAGCAAGTGAACAGA	0.200
4-2	NT_187032.1	82068280–82068580	F: [FAM]-AAGCTTCTCTGGCCATTTGA R: GTTCATAAACTTCAAGCAATGACA	0.125
6-7	NT_039353.8	51601265–51601685	F: [FAM]-AGTCCACCCAGTGCATTCTC R: GTTTCTTCATGTGGCTGGTATGCTGTT	0.075
9-2	NT_039474.8	74395400–74395000	F: [VIC]-GGATTGCCAAGAATTTGAGG R: GTTTCTTTCCTGAGTTGTGGACAGGGTTA	0.080
15-3	NT_039617.8	4930200–4930500	F: [NED]-TCTGGGCGTGTCTGTCATAA R: GTTTCTTTTCTCAGGGAGGAGTGTGCT	0.060
6-4	NT_039360.8	142021975–142022270	F: [NED]-TTTGCAACAGCTCAGTTTCC R: GTTTCTTAATCGCTGGCAGATCTTAGG	0.100
12-1	NT_039548.8	38480950–38481170	F: [VIC]-CAAAATTGTCATTGAACACATGTAA R: GTTTCTTTCAATGGTCAAGAAATACTGAAGTACAA	0.200
5-5	NT_109320.5	112641540–112641820	F: [PET]-CGTTTTACCTGGCTGACACA R: GTTTCTTGGTTTAAAACTCAATACCAAACAA	0.300
X-1	NT_039706.8	110959842–110960080	F: [PET]-GGATGGATGGATGGATGAAA R: GTTTCTTAAGGTATATATCAAGATGGCATTATCA	0.300
D8S1106	NT_167187.1	12835860–12836150	F: [VIC]-GTTTACCCCTGCATCACTGG R: GTTTCTTTCAGAATTGCTCATAGTGCAAGA	0.150
D4S2408	NT_006316.16	31304210–31304514	F: [NED]-TCATTTCCATAGGGTAAGTGAAAA R: GTTTCTTGCCATGGGGATAAAATCAGA	0.200

Mouse chromosomal locations (bp) are based on the current NCBI 38.1 mouse build. The chromosomal locations for human STR markers D8S1106 and D4S2408 (in bold) are based on the current NCBI 37.3 build. Primer concentrations listed are final concentrations of forward and reverse primers in a 20 μL reaction volume. Primer concentrations were determined empirically based on peak height, DNA concentration, and number of cycles in the PCR program