Table 1.
STR marker | GenBank accession # | Location on chromosome (bp) | Primers (5′–3′) | Primer μM |
---|---|---|---|---|
18-3 | NT_039674.8 | 60271556–60271705 |
F: [FAM]-TCTTTCTCCTTTTGTGTCATGC R: GTTTCTTGCTAAATAACTAAGCAAGTGAACAGA |
0.200 |
4-2 | NT_187032.1 | 82068280–82068580 |
F: [FAM]-AAGCTTCTCTGGCCATTTGA R: GTTCATAAACTTCAAGCAATGACA |
0.125 |
6-7 | NT_039353.8 | 51601265–51601685 |
F: [FAM]-AGTCCACCCAGTGCATTCTC R: GTTTCTTCATGTGGCTGGTATGCTGTT |
0.075 |
9-2 | NT_039474.8 | 74395400–74395000 |
F: [VIC]-GGATTGCCAAGAATTTGAGG R: GTTTCTTTCCTGAGTTGTGGACAGGGTTA |
0.080 |
15-3 | NT_039617.8 | 4930200–4930500 |
F: [NED]-TCTGGGCGTGTCTGTCATAA R: GTTTCTTTTCTCAGGGAGGAGTGTGCT |
0.060 |
6-4 | NT_039360.8 | 142021975–142022270 |
F: [NED]-TTTGCAACAGCTCAGTTTCC R: GTTTCTTAATCGCTGGCAGATCTTAGG |
0.100 |
12-1 | NT_039548.8 | 38480950–38481170 |
F: [VIC]-CAAAATTGTCATTGAACACATGTAA R: GTTTCTTTCAATGGTCAAGAAATACTGAAGTACAA |
0.200 |
5-5 | NT_109320.5 | 112641540–112641820 |
F: [PET]-CGTTTTACCTGGCTGACACA R: GTTTCTTGGTTTAAAACTCAATACCAAACAA |
0.300 |
X-1 | NT_039706.8 | 110959842–110960080 |
F: [PET]-GGATGGATGGATGGATGAAA R: GTTTCTTAAGGTATATATCAAGATGGCATTATCA |
0.300 |
D8S1106 | NT_167187.1 | 12835860–12836150 |
F: [VIC]-GTTTACCCCTGCATCACTGG R: GTTTCTTTCAGAATTGCTCATAGTGCAAGA |
0.150 |
D4S2408 | NT_006316.16 | 31304210–31304514 |
F: [NED]-TCATTTCCATAGGGTAAGTGAAAA R: GTTTCTTGCCATGGGGATAAAATCAGA |
0.200 |
Mouse chromosomal locations (bp) are based on the current NCBI 38.1 mouse build. The chromosomal locations for human STR markers D8S1106 and D4S2408 (in bold) are based on the current NCBI 37.3 build. Primer concentrations listed are final concentrations of forward and reverse primers in a 20 μL reaction volume. Primer concentrations were determined empirically based on peak height, DNA concentration, and number of cycles in the PCR program