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Thermal stabilitya of duplexes between tandem probes and their components with fcDNA targetb.
| Tandem | IVb + IIb | IVb + IIa | IVa + IIb | IVa + IIa | IVb + II | IV + IIb | IVa + II | IV + IIa | IV + II | II |
|---|---|---|---|---|---|---|---|---|---|---|
| N pyr c | 4 | 3 | 3 | 2 | 2 | 2 | 1 | 1 | 0 | — |
| T m, °C | 51.2 | 50.8 | 49.3 | 49.7 | 45.8 | 49.7 | 53.4 | 48.8 | 53.3 | 42.0 |
aConditions of thermal stability determination are listed in experimental section. bFor fcDNA sequence see Figure 1. cNumber of pyrene residues at the junction.
