. Author manuscript; available in PMC: 2014 Jan 9.

Published in final edited form as: J Comp Neurol. 2012 Jan 1;520(1):10.1002/cne.22685. doi: 10.1002/cne.22685

TABLE 1.

Injection Parameters

		HNM1	HNM2	HNM3
	Number of survival days	7	7	5
SC injections	Number of penetrations per hemisphere	4	4	4
	Number of injections per penetration (depths)*	2 (−1, −1.5 mm)	2 (−0.5, −1.5 mm)	3 (−0.5, −1, & −1.5 mm)
	Amount of virus or dye per injection (concentration)	1 μl RV (4.16× 10⁸iu/ml)	1 μl RV (8.53× 10⁹iu/ml)	1 μl RD (10%)
MT injections	Number of penetrations per hemisphere	n/a	n/a	2
	Number of injections per penetration	n/a	n/a	5
	Amount of virus or dye per injection	n/a	n/a	0.5 μl RV (6.44× 10⁸ iu/ml)

Injection depth from the superior collicular surface. RV, G-deleted GFP rabies virus; RD, tetramethylrhodamine dextran.