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Inhibition of MCP-1, IL-6, and MMP-9 levels by CMC 2.5 in PBMC cells. PBMC cells (5 × 105 cells/well) were cultured in serum-free media (37°C, 95% air, 5% CO2) for 18 hours with LPS (P. gingivalis, 50 ng/mL) or vehicle alone. CMC 2.5 was added at a final concentration of 5 μM. Conditioned medium was analysed for MCP-1 or IL-6 or MMP-9 by ELISA. Each value represents the mean of 3 cultures ± S.E.M. *P < 0.05.
