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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Dev Biol. 2013 May 8;380(1):10.1016/j.ydbio.2013.04.033. doi: 10.1016/j.ydbio.2013.04.033

Fig. 5. Zic2a and zic2b regulate neural crest migratory onset.

Fig. 5

Morphant (A–D) or overexpressing embryos (E–F) stained by ISH for sox10. (A) At 18S, sox10 is expressed in NC cells, which have exited the neural tube in control morphants (17/21, 3 experiments). (B,C) Single knockdown of Zic2a or Zic2b results in NC cells abnormally localized to the dorsal neural tube (8/12 zic2a morphants, 2 exp.; 14/32 zic2b morphants, 3 exp.). (D) Double Zic2 depletion causes a similar buildup of sox10-positive cells on the dorsal neural tube (7/10 embryos, 2 exp.). (E,F) Transgenic embryos were heat shocked at 10hpf to induce Zic2aYFP expression. Embryos expressing the zic2aYFP fusion protein have wild-type localization of NC cells, but increased sox10 expression (20/30, 2 exp.). (G) Quantitative real-time PCR for sox10 in 24hpf embryos after 10hpf HS. Relative abundance of sox10 transcript averaged across two biological replicates. A–F are dorsal views, anterior to the left.