Fig. 5.

Sct stimulates an acute FFA release in vivo through SctR. Stimulation of lipolysis by Sct was detected by measuring changes in the levels of FFAs in circulation after ip injection of Sct. Blood was collected from the tail vein for measurement of FFAs. A: Sct (0.5 mg/kg, ip) induced an acute lipolytic response in Wt mice (n = 9) up to 15 min after injection. *P < 0.05. B: The same dose of ip Sct significantly augments FFA concentrations in Wt (n = 8) and Sct^−/− (n = 9) mice, but not in SctR^−/− (n = 7) mice. *P < 0.0005, **P < 0.0001 from control. C: CL-316243 (0.1 mg/kg), a β₃-AR agonist, had similar effects in Wt and SctR^−/− mice (n = 8 each). *P < 0.005 from control.