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. 2014 Feb;55(2):201–213. doi: 10.1194/jlr.M040394

Fig. 5.

Fig. 5.

Effect of Tan on the expression of HO-1 in macrophages in vitro and in vivo. THP-1-derived macrophages (A) or primary human macrophages (B) were incubated with the indicated concentrations of Tan for 12 h and whole cell lysates were subjected to Western blotting to determine the protein expression of HO-1, with α-tubulin as the loading control. *P < 0.05 versus vehicle-treated group. C: Six-week-old ApoE−/− mice fed a high-cholesterol diet were dosed daily with Tan (30 mg/kg/day, i.g.) or vehicle (CMC-Na) for 12 weeks. The peritoneal macrophages were isolated, lysed, and subjected to Western blotting to evaluate the protein levels of HO-1 and α-tubulin. *P < 0.05 versus. vehicle-treated group. D: HO-1 immunohistochemical staining (red) of the aortic sinuses from ApoE−/− mice receiving Tan or vehicle treatment. Macrophages were stained with Mac3 (green) and nuclei were stained with DAPI (blue). E: THP-1 macrophages were incubated with the indicated concentrations of Tan for 4 h, then the nuclear extracts were subjected to Western blotting to determine the protein expression of Nrf2 and histone H1. *P < 0.05 versus vehicle-treated group. F: THP-1 macrophages were transfected with Nrf2 siRNA for 24 h, followed by Tan (10 μM) incubation for an additional 12 h. The expression of HO-1 or α-tubulin was examined by Western blotting. *P < 0.05 versus vehicle-treated group, #P < 0.05 versus Tan-treated alone group.