Figure 1.

Mitochondrial fraction purity from control and CR mice as assessed by the distribution of the activities of (A) citrate synthase and (B) lactate dehydrogenase. Enzyme activities were determined in the three mitochondrial fractions, as well as the cytosol, as described in the text, and expressed as μmol/min/mg protein. All values were presented as mean ± SEM (n = 6). Bars within a group, and between groups, that do not share the same letter are significantly different (P < 0.05). Black bar, M1; Gray bar, M3; Light gray bar, M10; White bar, cytosol.