Skip to main content
. Author manuscript; available in PMC: 2014 Jan 9.
Published in final edited form as: Cancer Res. 2009 Mar 17;69(7):10.1158/0008-5472.CAN-08-2281. doi: 10.1158/0008-5472.CAN-08-2281

Figure 5.

Figure 5

Chemokines produced by a subset of melanoma cell lines can attract human CD8+ effector T cells in a xenograft setting in vivo. A, supernatants from a series of melanoma cell lines were examined for chemokine content using a protein array. Melanoma cell line M537 showed a more diverse chemokine production profile. The indicated chemokines are GRO (1), IL-8 (2), CTACK (3), CXCL16 (4), IP-10 (5), MCP-1 (6), MIG (7), MIP-1α (8), MIP-1β (9), PARC (10), and RANTES (11). Each sample is represented in duplicate. The four top left corner spots and two bottom right corner spots represent loading controls. B, human melanoma cell lines were implanted s.c. into NOD/scid mice. Once they grew to a solid tumor, CD8+ effector T cells were prepared from normal human donors, labeled with CFSE as a fluorescent indicator, and injected i.v. The indicated tissues were then harvested and analyzed by flow cytometry for the presence of labeled T cells versus side scatter. Similar results were seen in at least two independent experiments.