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. 2013 Dec 19;2(6):e000376. doi: 10.1161/JAHA.113.000376

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© 2013 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Figure 6. — Pax‐7 and MyoD co‐localization at day 21 after ischemia. Sections of ischemic muscle recovered from saline‐, AG‐, and UnAG‐treated mice at day 21 post surgery and stained for Pax‐7 (green), MyoD (red), and DAPI (blue). Yellow arrows indicate merged Pax7⁺/MyoD⁺ cells. Scale bar: 40 μm (×40 magnification). Quantification of Pax7⁺/MyoD⁺cells in ischemic muscles of treated animals at day 21 is reported. Data are expressed as mean±SEM (n=9 each group at day 7 and at day 21) of Pax7⁺/MyoD⁺ cells per field (×40 magnification) (***P<0.001 ischemic muscles of UnAG‐ vs AG‐ and saline‐treated mice at day 21). AG indicates acylated ghrelin; UnAG, unacylated ghrelin.