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. 2013 Dec 19;2(6):e000355. doi: 10.1161/JAHA.113.000355

Figure 6.

Figure 6.

HK2 modulates reactive oxygen species (ROS) levels by increasing pentose phosphate pathway flux. A, Mean ROS levels as measured by 2′,7′‐di‐chlorofluorescein (DCF) fluorescence in AdβGal‐ and AdHK2‐infected NRVMs treated with either vehicle or 25 μmol/L phenylephrine for 48 hours (n=4). B, Mean ROS levels as measured by DCF fluorescence in CONsi‐ and HK2‐transfected NRVM treated with either vehicle or 25 μmol/L phenylephrine for 48 hours (n=4). C, Mean ROS levels as measured by DCF fluorescence in AdβGal‐ and AdHK2‐infected NRVMs treated with vehicle or 50 μmol/L H2O2 for 1 hour (n=4). D, Cell death, as measured by Sytox Green, in AdβGal‐ and AdHK2‐infected NRVMs treated with vehicle or 50 μmol/L H2O2 for 1 hour (n=6). E, Reduced glutathione (GSH) levels in cardiac lysates from NTG and TG mice (n=6). F, GSH levels in AdβGal‐ and AdHK2‐infected NRVMs (n=3). G, Glucose‐6‐phosphate dehydrogenase (G6PDH) enzymatic activity in AdβGal‐ and AdHK2‐infected NRVMs treated with vehicle or 25 μmol/L of the G6PDH inhibitor dehydroepiandrosterone (DHEA, n=4). H, Mean phenylephrine (PE)‐induced increase in ROS levels in AdβGal‐ and AdHK2‐infected NRVMs treated with vehicle or 25 μmol/L DHEA (n=4). I, Mean PE‐induced increase in NRVM cell area in AdβGal‐ and AdHK2‐infected NRVMs treated with vehicle or 25 μmol/L DHEA (n=5). Error bars represent SEM with *P<0.05 vs Vehicle and †P<0.05 vs AdβGal or CONsi. AdβGal indicates adenovirus expressing β‐galactosidase; HK2, hexokinase‐2; NRVM, neonatal rat ventricular myocytes; NTG, non‐transgenic; SEM, standard error of the mean; TG, transgenic.