Skip to main content
. 2013 Dec 19;2(6):e000548. doi: 10.1161/JAHA.113.000548

Figure 2.

Figure 2.

Specificity of aP2‐cre–mediated Vhl recombination in adipocytes. A, No significant Vhl recombination was found in heart, liver, and skeletal muscle. DNA was made from indicated tissues. The floxed Vhl alleles before (2‐Lox) and after (1‐Lox) recombination were detected by PCR as described in Methods. B, No significant Vhl recombination was found in macrophages. Genomic DNA was prepared from interscapular fat pad (BAT), perigonadal fat pad (WAT), or thioglycollate‐induced peritoneal macrophages. The recombination product (Vhl 1‐lox) was measured by quantitative PCR. C, Specific accumulation of HIF‐1α and HIF‐2α proteins in both WAT and BAT of fatVHLko mice was confirmed by Western blotting. No accumulation of HIF‐1α was observed in skeletal muscle and liver. No accumulation of HIF‐2α was observed in skeletal muscle. Note that HIF‐2α was constitutively expressed in liver. D and E, Visceral adiposity (WAT) in fatVHLko mice was generally reduced in comparison to their wild‐type littermates, whereas weight of interscapular fat (BAT) did not change significantly (6 to 10 weeks old). *P<0.05 (t‐test) compared with fatVHLwt, n=3 (male), n=5 (female). BAT indicates brown adipose tissue; HIF, hypoxia‐inducible factor; KO, knockout; PCR, polymerase chain reaction; VHL, von Hippel‐Lindau; WAT, white adipose tissue; WT, wild type.