Figure 3.

Gross phenotypes and hematological parameters of fatVHLko mice. A, Body weight comparison of 6‐ to 8‐week‐old mice, n=7 to 9 per group. Hematocrit (B), hemoglobin concentrations (C), and red blood cell (RBC) counts (D) of the peripheral blood of fatVHLko mice and wild‐type littermates (n=3 each, 6‐ to 8‐week‐old). No significant statistical differences were found. (E) Increased accumulation of HIF‐2α was found in adipose tissue only by Western blot using tissue homogenates from fatVHLko (KO) and wild‐type littermate controls (WT) with HIF‐1β as loading control. Results were validated in 3 pairs of KO and WT mice. F, Lack of fat accumulation (Oil Red O staining) in frozen sections of heart, liver, and skeletal muscle from fatVHLko and wild‐type mice. BAT and liver from a hepatocyte‐specific Vhl knockout mouse were used as positive controls. BAT indicates brown adipose tissue; HIF, hypoxia‐inducible factor; SM, skeletal muscle; VHL, von Hippel–Lindau.