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. 2013 Dec 19;2(6):e000548. doi: 10.1161/JAHA.113.000548

Table 1.

Primers for SYBR Green PCRs

Gene RefSeq Primers
Adm NM_009627 Forward: CACCCTGATGTTATTGGGTTCA Reverse: TTAGCGCCCACTTATTCCACT
Angptl3 NM_013913 Forward: GCCCTCCCAGAGCACACAGAC Reverse: TCCACCACCAGCCACCTGAGT
Apoa5 NM_080434 Forward: GACTACTTCAGCCAAAACAGTTGGA
Reverse: AAGCTGCCTTTCAGGTTCTCCT
Apoc4 NM_007385 Forward: TGTCTACAGAAAGCCTGAGCCCCAReverse: CTCCAGGGCCCCAGAACCACT
Apom NM_018816 Forward: CCATCCGCACGAAAAGTGGGGTReverse: TGTCTGGGCGCCCTTCAGTTCT
Ccl2 NM_011333 Forward: GCCAGCTCTCTCTTCCTCCACCAReverse: GGGCGTTAACTGCATCTGGCTGA
Ccl3 NM_011337 Forward: ATGGAGCTGACACCCCGACTGCReverse: TCAGGAAAATGACACCTGGCTGGGA
Ccl7 NM_013654 Forward: CAACCAGATGGGCCCAATGCATCCReverse: AGCTTCCCAGGGACACCGACT
Ccl8 NM_021443 Forward: TGGGCCAGATAAGGCTCCAGTCAReverse: AACCACAGCTTCCATGGGGCACT
Col1a1 NM_007742 Forward: AGGCTTCAGTGGTTTGGATGReverse: CACCAACAGCACCATCGTTA
Ctgf NM_010217 Forward: CAAAGCAGCTGCAAATACCAReverse: GGCCAAATGTGTCTTCCAGT
Egln3 NM_028133 Forward: AGGCAATGGTGGCTTGCTATCReverse: GCGTCCCAATTCTTATTCAGGT
Fgf10 NM_008002 Forward: TTTGGTGTCTTCGTTCCCTGTReverse: TAGCTCCGCACATGCCTTC
Gpd2 NM_010274 Forward: GAAGGGGACTATTCTTGTGGGTReverse: GGATGTCAAATTCGGGTGTGT
Glut3 NM_011401 Forward: ATGGGGACAACGAAGGTGACReverse: GTCTCAGGTGCATTGATGACTC
Gyk NM_008194 Forward: GGGTTGGTGTGTGGAGTCTTGReverse: GATTTCGCTTTCTTCAGCATTGA
Hmox1 NM_010442 Forward: GCCACCAAGGAGGTACACATReverse: GCTTGTTGCGCTCTATCTCC
Hprt NM_013556 Forward: AGTGTTGGATACAGGCCAGACReverse: CGTGATTCAAATCCCTGAAGT
Ifi27l2a NM_029803 Forward: CAGCTGCCTCCATAGCAGCCAAGReverse: GGAGAGTCCAAGGACCCCTGCT
Lep NM_008493 Forward: GGGCTTCACCCCATTCTGAReverse: AGGCTGGTGAGGACCTGTTG
Myh6 NM_010856 Forward: AAGGCAAGGCCAAGAACGCCReverse: AGCTCAGCCTTGGCCTCCAT
Myh7 NM_080728 Forward: ATGAGCAAGCCCTGGGCAGTReverse: AGAGCGCAGCTTCTCCACCT
Nppa NM_008725 Forward: AACTGAGGGCTCTGCTCGCTReverse: TTCGGTACCGGAAGCTGTTGC
Nfatc1 NM_016791 Forward: TCGAGTGTTCCCAGCGGTCAReverse: GCACAGGTCCCGGTCAGTCT
Nfatc2 NM_010899 Forward: ATCCGCGTGCCCGTGAAAGReverse: TCTTGATGGCAGGGACTGGGT
Nfatc3 NM_010901 Forward: GAGTGCTCTCAGCGATCTGCTCReverse: TCCTTCAGCCTCCCAATGAGGTC
Nfatc4 NM_023699.3 Forward: CACCCTGGAGGAAGTGAGTGAGATCReverse: GGGGCTTCGGGGACCACTAC
Osf2 NM_015784 Forward: CTGGTATCAAGGTGCTATCTGCReverse: AAACATGGTCAATAGGCATCACT
Plgf NM_008827 Forward: TCTGCTGGGAACAACTCAACAReverse: GTGAGACACCTCATCAGGGTAT
Ppargc1a NM_008904 Forward: GGACAGTCTCCCCGTGGATReverse: TCCATCTGTCAGTGCATCAAATG
Rcan1‐1 NM_001081549 Forward: GAGGAGGTGGATCTGCAGGACCTReverse: CACAGGCCGTCCACGAACACG
Rcan1‐4 NM_019466 Forward: TCGTTAAGCGTCTGCCCCGTGReverse: GCCACACAAGCAATCAGGGAGCT
Serpine1 NM_008871 Forward: TGCAACCCTGGCCGACTTCAReverse: CGGGCTGAGATGACAAAGGCTGT
Thbs1 NM_011580 Forward: GGGGAGATAACGGTGTGTTTGReverse: CGGGGATCAGGTTGGCATT
Vcam1 NM_011693 Forward: TGCCGAGCTAAATTACACATTGReverse: CCTTGTGGAGGGATGTACAGA
Vegfd NM_010216 Forward: TTGAGCGATCATCCCGGTCReverse: GCGTGAGTCCATACTGGCAAG
18S rRNA NR_003278 Forward: CGGACAGGATTGACAGATTGReverse: CAAATCGCTCCACCAACTAA
Nd2 Genomic, mitochondrial Forward: CCCATTCCACTTCTGATTACCReverse: ATGATAGTAGAGTTGAGTAGCG
PolB Genomic, nuclear Forward: CCCTCTTCCTCTTCATTATATCTCCReverse: CCTCAACTCCACCACAACAC

Specificity of the primers was validated using NCBI Primer‐BLAST.