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. 2013 Dec 19;2(6):e000439. doi: 10.1161/JAHA.113.000439

Figure 4.

Figure 4.

Confocal microscopic images depicting MIF deficiency‐induced accentuation of doxorubicin (DOX)‐induced cardiomyocyte apoptosis. A through X, Frozen myocardial sections from WT and MIF−/− mice treated with either saline or DOX were stained with desmin (red), TUNEL (green), and nucleus with DAPI (blue). Data were from 3 independent experiments each with 3 mice per group (for a total of 9 mice per group). Arrows denote cardiomyocyte apoptosis (the co‐localization of desmin, TUNEL and DAPI staining); Y, Quantitative analysis of apoptosis using TUNEL staining 7 days after DOX injection (≈50 fields from 3 mice per group). Mean±SEM, n=3 mice per group, *P<0.05 vs WT group, #P<0.05 vs WT DOX group, †P<0.05 vs MIFko DOX group. DAPI indicates 4′,6‐diamidino‐2‐phenylindole; ko, knockout; MIF, macrophage migration inhibitory factor; rmMIF, recombinant mouse macrophage migration inhibitory factor; TUNEL, terminal dUTP nick end‐labeling; WT, wild type.