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. 2013 Dec 19;2(6):e000439. doi: 10.1161/JAHA.113.000439

Figure 5.

Figure 5.

Mitochondrial morphology, function, and respiration in hearts from WT and MIF−/− mice treated with saline or doxorubicin (DOX). Representative transmission electron microscopy (TEM) image of left ventricular tissues (A through D, scale bar=500 nm); E, quantitative analysis of the percentage of mitochondrial area (≈50 mitochondria per group); representative images of JC‐1 staining of cardiomyocytes (F through K, scale bar=30 μm); L, quantitative analysis of the red/green fluorescence ratio (≈50 cardiomyocytes from 3 mice per group); mitochondrial respiration (M, CCO activity/complex IV; N, complex I/III; O, complex II/III). M1: Healthy mitochondria; M3: degenerating mitochondria; Sar: sarcomere; arrows denote double membrane vacuoles. Mean±SEM, n=50 cardiomyocytes per group, *P<0.05 vs saline group, #P<0.05 vs WT DOX group, †P<0.05 vs MIFko DOX group. CCO indicates cytochrome c oxidase; ko, knockout; MIF, macrophage migration inhibitory factor; rmMIF, recombinant mouse macrophage migration inhibitory factor; WT, wild type.