Figure 6.

TLR2 is important for inflammatory responses in the heart during pressure overload. A through D, Mice were analyzed at 2 weeks after TAC or sham operation. A, NF‐κB activity in the heart (n=4). Free p65 levels in the nucleus were measured. Values were normalized to protein levels. B, mRNA expression levels of IL‐1β (Il1β), IL‐6 (Il6) and TNF‐α (Tnfα) in the heart (n=4). Expression levels of each gene were normalized to those of GAPDH (Gapdh). C, Macrophage infiltration. Macrophage density was assessed in heart sections with anti–Mac‐3 staining (n=4). Scale bars=20 μm. D, Monocyte chemotactic protein‐1 (Mcp1) mRNA expression (n=4). Expression levels of Mcp1 mRNA were normalized to those of GAPDH (Gapdh) mRNA. *P<0.05 vs sham; ^†P<0.05 vs Tlr2^+/+ mice. IL indicates interleukin; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NF, nuclear factor; TAC, transverse aortic constriction; TLR, Toll‐like receptor; TNF, tumor necrosis factor; WT, wild‐type.