Table 1. Effects of pH and buffering on terminal morphology and statistical analysis for experimentally matched RLS data shown in Figure 1.
| pH | Buffer or pH
adjusted by |
Median
RLS |
RLS vs.
YPD P |
Budded | Unbudded | %B | RLS B | %U | RLS U | B&U vs. YPD
P value |
|---|---|---|---|---|---|---|---|---|---|---|
| 8.0 | NaOH | 28.5 | 0.7658 | 18 | 22 | 45.0 | 23 | 55.0 | 30.5 | 0.3711 |
| 7.8 | Tris buffer | 27 | 0.1754 | 23 | 17 | 57.5 | 27 | 42.5 | 26 | 1 |
| 7.0 | MOPS buffer | 30 | 0.4161 | 21 | 19 | 52.5 | 25 | 47.5 | 36 | 0.8224 |
| 7.0 | Tris buffer | 27 | 0.3653 | 21 | 18 | 53.8 | 25 | 46.2 | 28 | 0.8224 |
| 6.8 | YPD | 29 | - | 23 | 17 | 57.5 | 25 | 42.5 | 34 | - |
| 6.0 | MES buffer | 21.5 | 0.0165 | 13 | 27 | 32.5 | 16 | 67.5 | 22 | 0.0424 |
| 5.6 | Acetate buffer | 20.5 | 0.0896 | 15 | 25 | 37.5 | 18 | 62.5 | 25 | 0.1165 |
| 5.0 | HCl | 22.5 | 0.0218 | 11 | 29 | 27.5 | 18 | 72.5 | 23 | 0.0123 |
| 5.0 | Acetic acid | 28 | 0.8869 | 14 | 25 | 35.9 | 25 | 64.1 | 30 | 0.0722 |
| 3.0 | Citrate buffer | 25.5 | 0.3412 | 11 | 29 | 27.5 | 26 | 72.5 | 25 | 0.0123 |
RLS, median replicative lifespan; U, unbudded; B, budded.