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. Author manuscript; available in PMC: 2014 Jan 9.
Published in final edited form as: Oncogene. 2011 Jun 13;31(3):10.1038/onc.2011.227. doi: 10.1038/onc.2011.227

Figure 5.

Figure 5

UHRF2 influences expression of p16INK4a, p21Waf1/Cip1, p27Kip1 and pRB. (a) mRNA levels of p21Waf1/Cip1, p16INK4a and p27Kip1 were examined by real-time RT–PCR after knocking down UHRF2 in HCC1954 cells. The baseline for the cells infected with control shRNA was arbitrarily set as 1. (b) Protein levels of p21Waf1/Cip1, p16INK4a, p27Kip1 and pRB in HCC1954 cells stably expressing control shRNA, UHRF2 shRNA#1 or shRNA#2 were analyzed by western blot. The migration control for the hypophosporylated (p) form of RB protein is shown in Supplentmentary Figure S7. (c) Overexpression of UHRF2 in MCF10A cells results in reduced protein levels of p16INK4a, p21Waf1/Cip1 and p27Kip1, but not of pRB as determined by western blot.