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. Author manuscript; available in PMC: 2014 Jan 9.
Published in final edited form as: Leukemia. 2012 Sep 13;27(3):10.1038/leu.2012.269. doi: 10.1038/leu.2012.269

Figure 5.

Figure 5

MMSET binding to the miR-126* promoter is associated with KAP1 presence and chromatin modifications favoring gene repression. Chromatin from MMSET shRNA-inducible KMS11 cells grown with or without dox for 7 days was immunoprecipitated with anti-MMSET (a), anti-H3K9me3 (b), anti-KAP1 (c) or anti-acetyl histone H3 (d) antibodies. Immunoglobulin G (IgG) antibody was used as a negative control. Precipitated DNA was analyzed by real-time PCR using primers amplifying regions near the predicted miR-126* TSS and 10 kb upstream (10 kb Up). Results are presented as percentage of total input DNA precipitated. Values represent mean ± s.d. from two experiments.