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. 2013 Dec 27;8(12):e84690. doi: 10.1371/journal.pone.0084690

Figure 6. GS II-FITC lectin binding affinity of the indicated F. oxysporum strains.

Figure 6

Intensity of fluorescence emitted by GS II-FITC bound to cell surface was measured for 20,000 events in microconidia from wild type (wt), Δgnt2 mutant and cΔgnt2 complemented strain, using flow cytometer separation and fluorescence detection. (A) Fluorescence analysis using the auxiliary channel adjusted to allow discrimination of single cell population (H3 in the histograms and white columns in the graph) from aggregated cells population (H4 and stripped columns). The percentages of each cell population are represented for the three strains. (B) Histograms showing relative fluorescence determined by FL1 channel for single cells (H3) or aggregated cells (H4) from the three strains. Columns in the graph represent the relative fluorescence medium values observed for single cells (white) and aggregated cells (stripped). The standard errors from three independent experiments are indicated. (C) Morphological analyses of cell populations from the different strains by cytometer light scattering detection. Forward and side scattered light shape (FSC and SSC, respectively) is represented.