Skip to main content
. 2013 Dec 27;8(12):e84690. doi: 10.1371/journal.pone.0084690

Figure 9. Gnt2 co-localizes with Golgi sub-cellular compartment proteins.

Figure 9

(A) Light (left panels) and fluorescence (right panels) micrographs of germlings from the wild type (wt) and the Δgnt2 mutant, both harbouring the GFP::Gnt2 fusion protein, after 5 min treatment with (+) or without (-) Brefeldin A (BFA). Scale bars, 10 µm. (B) Enzymatic activities of sub-cellular fractions (1 to 14) obtained after velocity sucrose gradient ultracentrifugation of cell lysates from the indicated strains. Aliquots of the 10,000 g x 10-min pellet (P10) and the 160,000 g x 90-min pellet (P160) were also included in the analyses. Dashed line, sucrose concentration; black diamonds, NADPH cytochrome c reductase activity (endoplasmic reticulum marker); white diamonds, GDPase activity (Golgi marker). (C) Proteins contained within the indicated fractions were resolved by SDS-PAGE and detected by Western blotting analyses using anti-GFP or anti-Vps10p antibodies, as indicated. (D) Colocalization of GFP::Gnt2 (green) with the Golgi apparatus (red) as stained with BODIPY TR ceramide in the indicated strains. Bar, 10 μm.