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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Med. 2013 Dec 8;20(1):10.1038/nm.3416. doi: 10.1038/nm.3416

Figure 2. 13C spectroscopic imaging showing the spatial distribution of labeled glucose and lactate.

Figure 2

(a) Representative 13C MR spectra acquired from subcutaneous EL4 and LL2 tumors, brain, heart, liver, and kidneys 15 s after the injection of 0.35 mL 100 mM hyperpolarized [U-2H, U-13C]glucose. The lactate spectra are the sum of 4 transients collected over a period of 1 s, whereas a single transient was acquired for the glucose spectra. Flux of hyperpolarized 13C label was only observed between [U-2H, U-13C]glucose (63–99 p.p.m.) and lactate C1 (doublet at ~185 p.p.m.) in EL4 and LL2 tumors. (b) Representative chemical shift selective images obtained ~15 s after intravenous injection of 0.4 mL 200 mM hyperpolarized [U-2H, U-13C]glucose into an EL4 tumor-bearing mouse. The spatial distribution of glucose, urea and lactate are displayed as voxel intensities relative to their respective maxima. The 1H MR images, shown in gray scale, were used to define the anatomical location of the tumor (outlined in white). A urea phantom was included to serve as a reference. The color scales represent arbitrary linearly distributed intensities for the hyperpolarized images.