. Author manuscript; available in PMC: 2014 Jul 1.

Published in final edited form as: Nat Med. 2013 Dec 8;20(1):10.1038/nm.3416. doi: 10.1038/nm.3416

Table 1.

Glucose and lactate concentrations measured in tissue extracts.

		Natural abundance signals		[U-¹³C]Glucose signals
		Glucose	Lactate	Glucose	Lactate
20 s post-injection
EL4 (μmol g⁻¹ wt)	Untreated	0.42±0.06	20.7±3.0	0.13±0.02	0.49±0.10
	Treated	0.46±0.13	12.7±1.9^a	0.16±0.04	0.50±0.13
Blood (μmol mL⁻ ¹)	Untreated	3.3±0.1	3.3±0.4	1.8±0.1	n.d.
	Treated	3.2±1.0	3.3±0.6	3.8±0.9	n.d.
Liver (μmol g⁻¹ wt)	Untreated	11.4±1.1	7.2±1.3	1.7±0.4	0.20±0.06
	Treated	10.6±1.5	5.4±0.6	1.4±0.3	0.06±0.02 ^a
150 s post-injection
^EL4 (μmol g⁻¹ wt)	Untreated	1.4±0.3	20.0±1.0	0.40±0.07	1.9±0.4
	Treated	1.5±0.3	12.0±0.5 ^a	0.49±0.10	1.2±0.2
Liver (μmol g⁻¹ wt)	Untreated	9.2±3.2	6.0±0.3	1.1±0.1	0.31±0.01
	Treated	9.8±3.0	5.3±0.7	1.3±0.2	0.29±0.04

Tumors were freeze-clamped and extracted 20 s or 150 s after intravenous injection of 0.35 mL 100 mM [U-¹³C]glucose. n = 5 for EL4 and liver samples (post 20 s) and n = 4 for EL4 and liver samples (post 150 s), n = 3 for blood plasma samples. Mean ± S.E.M.;

p < 0.05; n.d., not detected.