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. 2014 Jan 9;9(1):e81730. doi: 10.1371/journal.pone.0081730

Figure 2. HCV core protein promoted cell growth, G1/S transition and colony formation efficacy of HepG2 cells via its down-regulation on miR-152.

Figure 2

HepG2 cells were transfected with double-stranded miR-152 mimics, single-stranded miR-152 inhibitor, or their relative negative control RNA at a final concentration of 5 nM with a HiPerFect Transfection Reagent kit as indicated for 48 h, and were collected for preparation of MTT, flow cytometry and colony formation assay. (A) Results of MTT assay. (B) Results for cell cycle profiles. (C) Results for colony formation assay. Data are shown as means and standard deviations from triplicate experiments. * P<0.05, ** P<0.01. (Advirus-Hcv: Adenovirus expressing the HCV core protein; Advirus-Ctrl: Adenovirus expressing the EGFP control protein; inh NC: scrambled oligonucleotides as the negative control for miR-152 inhibitor; inh-152: miR-152 inhibitor; miR NC: scrambled oligonucleotides as the negative control for miR-152 mimics; miR-152: miR-152 mimics; HCV+ miR-152: Adenovirus expressing the HCV core protein+miR-152 mimics).