Table 1. MuSK autoantibodies positive patient sera listed according to RIA values.
Patient serum | RIA [pmol/L] | HEp-2 M4+ESM | HEp-2 M4−ESM | p values | |||||
X¯ | ±SD | FI | X¯ | ±SD | FI | p1 | p2 | ||
1 | 343400 | 0.288 | 0.048 | + | 0.074 | 0.011 | + | 2.8E-5 | 2.0E-4 |
2 | 179600 | 0.371 | 0.076 | +++ | 0.162 | 0.111 | ++ | 4.0E-4 | 2.1E-4 |
3 | 2300 | 0.425 | 0.129 | +++ | 0.116 | 0.025 | ++ | 2.8E-5 | 2.7E-4 |
4 | 2205 | 0.280 | 0.114 | +++ | 0.066 | 0.019 | ++ | 1.3E-4 | 4.3E-4 |
5 | 2189 | 0.288 | 0.094 | +++ | 0.075 | 0.013 | ++ | 1.2E-4 | 4.5E-4 |
6 | 2176 | 0.290 | 0.135 | +++ | 0.063 | 0.008 | ++ | 1.2E-4 | 2.8E-4 |
7 | 2107 | 0.368 | 0.063 | +++ | 0.084 | 0.004 | + | 1.2E-4 | 5.0E-4 |
8 | 1984 | 0.351 | 0.107 | ++ | 0.063 | 0.016 | + | 2.8E-5 | 1.6E-4 |
9 | 1920 | 0.240 | 0.116 | ++ | 0.047 | 0.024 | + | 1.2E-4 | 2.0E-4 |
10 | 1890 | 0.413 | 0.177 | + | 0.124 | 0.026 | + | 4.7E-4 | 3.7E-3 |
11 | 1861 | 0.467 | 0.019 | ++ | 0.084 | 0.003 | + | 1.2E-4 | 3.9E-4 |
12 | 1844 | 0.333 | 0.083 | ++ | 0.066 | 0.010 | ++ | 1.2E-4 | 4.5E-4 |
13 | 1788 | 0.302 | 0.089 | +++ | 0.052 | 0.002 | ++ | 1.2E-4 | 2.8E-4 |
14 | 1785 | 0.485 | 0.031 | +++ | 0.089 | 0.002 | + | 1.2E-4 | 3.4E-4 |
15 | 1766 | 0.259 | 0.055 | ++ | 0.062 | 0.001 | ++ | 1.2E-4 | 3.4E-4 |
16 | 1752 | 0.277 | 0.059 | +++ | 0.060 | 0.011 | ++ | 1.2E-4 | 3.9E-4 |
17 | 1750 | 0.428 | 0.038 | +++ | 0.082 | 0.017 | ++ | 1.2E-4 | 4.3E-4 |
18 | 1729 | 0.333 | 0.126 | ++ | 0.064 | 0.005 | + | 1.2E-4 | 6.5E-3 |
19 | 1641 | 0.434 | 0.069 | +++ | 0.078 | 0.031 | ++ | 1.2E-4 | 3.9E-4 |
20 | 1592 | 0.469 | 0.064 | +++ | 0.088 | 0.028 | + | 1.2E-4 | 3.4E-4 |
21 | 1592 | 0.333 | 0.039 | + | 0.079 | 0.008 | +/− | 2.8E-5 | 1.2E-4 |
22 | 1491 | 0.268 | 0.070 | +++ | 0.063 | 0.022 | + | 2.2E-3 | 0.0997 |
23 | 837 | 0.105 | 0.063 | + | 0.019 | 0.001 | +/− | 1.1E-3 | 4.2E-3 |
24 | 791 | 0.255 | 0.120 | +/− | 0.022 | 0.013 | +/− | 2.2E-4 | 4.1E-3 |
25 | 580 | n.v. | − | n.v. | − | n.a. | n.a. | ||
26 | 194 | n.v. | − | n.v. | − | n.a. | n.a. | ||
27 | 170 | n.v. | − | n.v. | − | n.a. | n.a. | ||
28 | 112 | n.v. | − | n.v. | − | n.a. | n.a. | ||
29 | 110 | n.v. | − | n.v. | − | n.a. | n.a. | ||
30 | 90 | n.v. | − | n.v. | − | n.a. | n.a. | ||
31 | 87 | 0.348 | 0.115 | + | 0.054 | 0.010 | +/− | 2.8E-5 | 2.1E-3 |
32 | 80 | n.v. | − | n.v. | − | n.a. | n.a. | ||
33 | 79 | n.v. | − | n.v. | − | n.a. | n.a. | ||
34 | 70 | n.v. | − | n.v. | − | n.a. | n.a. |
The table provides data from Figure 4. MuSK autoantibody positive patient sera are shown for HEp-2 M4+ESM and HEp-2 M4−ESM. The sera are sorted to descending RIA values (RIA cut off: >50 pmol/L positive, >500 pmol/L strong positive). The value X¯ describes the mean value of positive cells related to total cell number which is combined with standard deviation (±SD) and semi quantitatively scored fluorescence intensity (FI). The p-values are given for HEp-2 M4+ESM compared to HEp-2 M4−ESM (p1) and HEp-2 M4−ESM compared to HEp-2 cells (p2). N.v.: not valuated because FI was negative, n.a.: not available due to negative FI.