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SPOTs array depicting the overlapping 15-aminoacid peptides covering the complete sequence of α-T, and probed with two different pools of serum from cow's milk allergic patients, a rabbit CMP-specific polyclonal serum and the 1D5 α-casein-specific mAb, followed with the appropriate conjugated secondary antibodies. Positive spots are shaded in grey boxes and recognized amino acids are bolded in the sequence depicted for spotted peptides.
