Figure 4. Schematic localization of the identified spots along the α-T sequence and sequence alignment analysis.

A) Analysis of the sequence alignment of α-T with IgE epitopes in bovine caseins (IEDB) shown as hit distribution of amino acid similitude per position along the sequence of α-T. Positions of α-T with high hit values (black columns) correlate with positive spots of the overlapping assay (spots 9, 10 and12); B) The sequence of α-T (1–234 aa) is depicted along with overlapped peptides, PA and PN (305–539 aa correspond to α). In silico predicted alpha-helix (white ovals) and beta-sheet (black arrows) zones are indicated. PA contains the amino acid positions with the highest hits and the α-helix and β strand secondary structures.