Table 1. Kinetic and equilibrium constants for complexes formed by α-casein, α subunit of β-conglycinin (α), α-T and PA with the 1D5 mAb, specific for α-casein.
kass (M−1 s−1) | kdiss (s−1) | KA (M−1) | KD (M−1) | |
Bovine α-casein | 1.05×104±0.14×104 | 8.88×10−4±0.22×10−4 | 1.18×107±0.13×107 | 8.44×10−8±1.48×10−8 |
α | 1.89×104±0.18×104 | 9.79×10−4±1.47×10−4 | 1.93×107±0.22×107 | 5.18×10−8±0.60×10−8 |
α-T | 3.14×104±0.44×104 | 2.70×10−3±0.40×10−3 | 1.16×107±0.06×107 | 8.60×10−8±0.44×10−8 |
PA | 6.92×103±0,48×103 | 1.70×10−3±1.15×10−3 | 4.06×106±2.75×106 | 2.46×10−7±1.67×10−7 |
kass: Association constant, kdiss: Dissociation constant, KA: Affinity constant, KD: Equilibrium Dissociation constant.