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. 2014 Jan 9;9(1):e84503. doi: 10.1371/journal.pone.0084503

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© 2014 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) SDS-PAGE analysis of Escherichia coli-expressed chCD132; M, molecular weight marker; lane 1, bacterial lysates of E. coli BL21 (DE3) transformed with pET28a; lane 2, bacterial lysates containing rchCD132. (B) Western blot analysis of rchCD132 recognized by 6 anti-chCD132 mAbs. (C) anti-chCD132 mAb C10 recognized by chCD132 expressed on the SMC surface using indirect immunofluorescencestaining (×10).