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. 2014 Jan 9;9(1):e84503. doi: 10.1371/journal.pone.0084503

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© 2014 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Bursacytes were incubated with FITC-labeled mouse anti-chicken CD3 mAb and PE-labeled mouse anti-chicken CD4 or CD8 mAb and APC-labeled mouse anti-chicken CD132 mAbs. Cells were counted on a Cytomics FC500 and analyzed using CELLQUEST software. Mock-infected SPF chickens were used as a negative control. All samples were assayed in triplicate.