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. 2014 Jan 9;9(1):e85491. doi: 10.1371/journal.pone.0085491

Figure 5. WcbK is important for LPS-biosynthesis, attenuates survival in blood, and promotes acid resistance.

Figure 5

(A) SDS-PAGE pattern of purified LPS after silver staining. Samples were isolated from C. fetus. subsp. fetus (Cff) 82-40 (lane 1), Cff ATCC 27374 (type B) (lane 2), wcbK mutant K19 (wcbK::Km) (lane 3) and K19 [pSW2] (wcbK in trans) (lane 4); C. fetus subsp. venerealis (Cfv) ATCC 19438 (lane 5) and Cfv 84-112 (lane 6). (B) Cff serum resistance assays. Strains were incubated either with EMEM (-), heat-inactivated (I) or active (A) human serum and colony forming units (CFU) were counted. Results shown are for Cff ATCC 27374, K19 and K19 [pWS2]. Cff 82-40 served as a type A comparator. (C) Same as in (B) but for better visualization, CFU/ml obtained after treatment with active serum are displayed separately. **p<0.002 (D) Acid resistance assays. Cff were incubated in PBS pH range 7.3 to 3.4, plated and CFU determined. Survival after exposure to different pH of the wild type, K19 and K19 [pSW2] was compared. (E) For better visualization, CFU/ml for the three strains after treatment with pH 3.4 were plotted separately. **p<0.003.