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. 2014 Jan 9;9(1):e85492. doi: 10.1371/journal.pone.0085492

Figure 1. I-sceI meganuclease-mediated transgenesis in lamprey embryos.

Figure 1

(A–C) Transient transgenic lamprey generated through injection of the linearised pm3285_cfos_EGFP plasmid showing mosaic GFP expression in the ectoderm and in neurons of the spinal cord at stage 23 (A), stage 25 (B) and at the ammocoete larval stage (C). (D–F) Lamprey embryo with GFP expression in the hindbrain and spinal cord at stage 23 (D), stage 24 (E) and stage 25 (F), obtained using the meganuclease assay with the pm3285 enhancer. (G–J) ‘Background’ GFP expression in the ectoderm of lamprey embryos at stage 19 (G), 21 (H), 23 (I) and 25 (J), driven by the cfos_IsceI_EFGP plasmid with no enhancer using the meganuclease method (embryos shown are different individuals). Abbreviations: ec, ectoderm; hb, hindbrain; ph, pharynx; sc, spinal cord.