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. 2014 Jan 9;9(1):e85492. doi: 10.1371/journal.pone.0085492

Figure 2. Reporter expression driven by CNEs 3285 and 3299 in lamprey and zebrafish embryos.

Figure 2

(A–B) GFP fluorescence in stage 26 transient transgenic lamprey embryos, generated by meganuclease-mediated transgenesis with the pm3285 (A) and dr3285 enhancers (B). GFP expression is seen in the cranial ganglia (arrowheads), hindbrain and spinal cord. (C–D) GFP fluorescence in the rostral hindbrain in stage 26 transient transgenic lamprey embryos generated by meganuclease-mediated transgenesis with the pm3299 (C) and dr3299 (D) enhancers. GFP expression driven by pm3285 (E) and dr3285 (F) in 54hpf F1 transgenic zebrafish embryos, created by Tol2 transgenesis. Expression in the cranial ganglia is seen for the elements from both zebrafish and lamprey (arrowheads). Expression is also seen in primary neurons of the hindbrain and spinal cord. (G–H) 54hpf F1 transgenic zebrafish embryos showing GFP expression driven by the pm3299 (G) and dr3299 enhancers (H). GFP expression in the hindbrain is driven by both elements, whilst only the zebrafish element up-regulates GFP in the neural crest. (I–J) Expression of Meis1/2a (I) and b (J) genes in stage 25 lamprey embryos, revealed by in-situ hybridisation. Arrowheads highlight expression in cranial ganglia. Abbreviations: glV2,3, trigeminal ganglion; gldIX, epibranchial ganglion of the glossopharyngeal nerve; gldX1, epibranchial ganglion of the vagus nerve; glf, facial ganglion; hb, hindbrain; nc, neural crest; ov, otic vesicle; ph, pharynx; pllg, posterior lateral line ganglion; sc, spinal cord.