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. 2014 Jan 9;9(1):e85571. doi: 10.1371/journal.pone.0085571

Figure 2. Ape/Ref-1 inhibition accelerates ATRA-induced growth arrest and apoptosis.

Figure 2

(A) U266 cells were transfected with specific siRNA vector (pSUPER Aper/Ref-1) or control vector (pSUPER Luc), and incubated for 48 h following by immunoblotting with antibodies againset Ape/Ref-1 or GAPDH. (B) After transfected with siRNA or control vectors for 24 h, U266 cells were treated with the indicated concentrations of ATRA for 72 h and the proliferation rates were monitored by CCK-8 assay. (C) Flow cytometry analysis of apoptosis in U266 cells transfected with siRNA vectors and then treated by ATRA at indicated concentrations for 72 h. Data are typical of three similar experiments. The percentage of Annexin V-FITC and/or PI positive cells was depicted with cytofluorometer quadrant graphs.