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. 2014 Jan 9;9(1):e85571. doi: 10.1371/journal.pone.0085571

Figure 3. Effect of ATRA on p38-MSK cascade activation in myeloma cells.

Figure 3

(A) U266 cells were treated with the indicated concentrations of ATRA for 2 h. Cell lysates were subjected to immunoblotting with antibodies to phosphorylated and total p38 as well as MSK1 proteins. (B) U266 cells were incubated with 1 µM of ATRA for the indicated periods. Phosphorylated p38 and MSK1 were measured by Western blotting. The experiment was repeated at least three times. The figure shows a representative result.