Figure 1. PAGE analysis of D. discoideum cell extract reveal the presence of three major bands.

Inositol phosphates were extracted from 10(WT) D. discoideum (AX2 strain) and the IP₆-Kinase (ip6k null) and phospholipase C mutant (plc null) grown at a density of 2–4×10⁶ _. About 30–40 microliters of neutralised cell extract (equivalent to 1/20 of the total volume) was resolved on 35.5% PAGE [26] and visualized with Toluidine blue (A) and DAPI staining (B). The figure shows the result of a representative experiment that was repeated three times.