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. 2014 Jan 9;9(1):e85469. doi: 10.1371/journal.pone.0085469

Figure 2. BMP-2 alone does not induce adipogenic differentiation in rat TSCs.

Figure 2

TSCs were incubated with (0, 10, 50, 100, or 200 ng/ml BMP-2 for 7 days. (A) Adipogenic differentiation detected by Oil Red O staining; (B) the areas stained with Oil Red O in the cells were assessed; (C) PPARγ mRNA levels determined by qRT-PCR. TSCs were incubated with 100 ng/ml BMP-2 for 0, 3, 7, or 10 days. (D) Adipogenic differentiation detected by Oil Red O staining; (E) the Oil Red O staining areas in the cells were assessed; (F) PPARγ mRNA levels determined by qRT-PCR. The mRNA levels were normalized using GAPDH. Results represent the mean ± SD.