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. 2014 Jan 9;9(1):e85469. doi: 10.1371/journal.pone.0085469

Figure 5. Knock down endogenous IGF-1 and BMP2 abolished PGE2-induced adipogenic differentiation.

Figure 5

Rat TSCs were treated with PGE2 (100 ng/ml) with or without BMP-2 shRNA or IGF-1 shRNA. (A) Levels of IGF-1 and BMP2 were determined by western blotting. β-actin was used as loading controls. (B) Adipogenic differentiation detected by Oil Red O staining; (C) The Oil Red O staining areas in the cells were assessed; (D) PPARγ mRNA levels determined by qRT-PCR. The mRNA levels were normalized using GAPDH. Results represent the mean ± SD. **P<0.01 with respect to TSCs without PGE2; #P<0.05 with respect to TSCs with PGE2.