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. 2014 Jan 9;9(1):e85715. doi: 10.1371/journal.pone.0085715

Figure 1. Infection of macrophages with L. major and generation of ROS.

Figure 1

(A, B) Resident or inflammatory macrophages from B6 mice were infected with L. major for 4 h, and washed. Cells were stained and percentages of infected macrophages (A) and number of parasites per 100 macrophages (B) were determined. (C) Resident or inflammatory B6 macrophages were loaded with DCFH-DA, washed, treated with medium (Uninfected) or with L. major for 4 h, and fluorescence was measured. Results indicate arbitrary units of fluorescence and are mean and SE of triplicates. *P<0.05; **P<0.01.