Skip to main content
. 2014 Jan 9;9(1):e85715. doi: 10.1371/journal.pone.0085715

Figure 6. Effects of antioxidants on ROS generation, JNK activation, KC release, and intramacrophagic parasite growth.

Figure 6

(A, B) Macrophages were loaded with DCFH-DA, washed and infected or not with L. major for 4 h in the presence of medium, antioxidants DFO (A), or NAC (B). Results indicate arbitrary units of fluorescence and are mean and SE of triplicates. (C, D) Macrophages were infected or not in the presence of medium, DFO (C), or NAC (D). After 4 h, the levels of JNK and p-JNK were determined by western blotting. (E, F) Macrophages were infected or not in the presence of medium, DFO (E), or NAC (F). The levels of KC were determined by ELISA after 20 h of infection. (G) Macrophages were infected overnight and cultured for additional 3 d in the presence of medium, DFO or NAC. Intracellular load of parasites was evaluated. Results are mean and SE of extracellular promastigotes produced. *P<0.05, **P<0.01.