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. 2014 Jan 9;10(1):e1003868. doi: 10.1371/journal.ppat.1003868

Figure 2. Endogenous CPSF6 restricts cell cycle-dependent HIV-1 CA mutants.

Figure 2

(A, B) HeLa cells transfected with either non-targeting siRNA or siRNA targeting CPSF6 were infected with RT-normalized VSV-G-pseudotyped GFP reporter viruses. Increase in viral infectivity of T54A by CPSF6 depletion was statistically significant (B; p<0.002) and different from that of T54A+N74D or T54A+A105T (p<0.001). (C) HeLa cells transfected with either non-targeting siRNA or siRNA targeting CPSF6 were infected with VSV-G-pseudotyped GFP carrying CA mutants that lost the cell cycle independence so that 0.5%-1% cells were infected with the viruses two days after infection. (D) An experiment similar to that described in C was carried out, except that all these CA mutants still maintain the cell cycle independence. Results are one representative experiment of at least two experiments. Standard deviations of a single triplicate experiment are indicated with the error bars.